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Explain Podcast

explain.pod

Explain Podcast

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Explain Podcast

explain.pod

Explain Podcast

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Explain Podcast

explain.pod

Explain Podcast

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Episodes of Explain Podcast

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Mixing up long and cheapChapters:00:00:00 Intro00:03:30 Florians history00:13:50 Q&A Ressources for Bioinformatics00:27:00 Long-read-tools.org00:35:42 ONT || PacBio00:57:00 Storage01:09:00 OutroUp-to-date, raw prices:https://albertv
Rolling in circles to make DNA yarnsChapters:00:00 Bioinformatics Upper Crust Tools04:04 STAR05:00 MGI15:00 Daniels first contact20:00 Brief Market Overview22:30 Short read market26:00 DFG High Costs Sequencing Projects Application29:
Who needs all bases anyway?Chapters:00:00 Phasing08:50 Not Sequencing, really14:00 Bionano Saphyr Chip26:00 Nabsys - going electric29:30 Tools available39:00 QuickGen with Iuliia: Transposons!Links:Small explainer by CAP: https://www
Chapters:00:00 Gambling15:00 Bubbles30:00 Holes57:30 One more thing1:07:00 Finale1:08:00 Finale of season 1!Eric Chow explaining single cell sequencing: https://www.youtube.com/watch?v=k9VFNLLQP8cOverview of things to consider: http
Getting the most out of MachinesChapters:00:00 PhiX14:30 low complexity19:30 UMIs32:10 FastQC43:00 MultiQC56:40 PycoQCPhiX concentrations for loading a validation run:https://knowledge.illumina.com/instrumentation/general/instrumenta
Pushing spaghetti through holesChapers:00:00 Intro11:15 Measuring Ions17:00 Quality23:00 Duplex24:30 MinION25:15 PromethION28:20 Methylation29:00 Direct RNA sequencing38:25 Basecalling46:00 Basecalling bias53:50 Sequencing proteins
Zipping rounds in zeptolitersChapters:00:00 Intro 07:00 Laser09:00 Zeptoliters13:30 Polymerase speed19:00 Error rate22:00 SMRT Cell25:45 ccs30:00 Fragment size34:30 Megaruptor38:20 Data flow47:00 SVs53:00 MAS Seq- Starter: https
Glued DNA bridges and rainbow clustersChapters:00:00-Flowcells03:40-DNA glued to glass09:00-Bridge amplification15:50-Cluster density and patterned Flowcells28:30-Illumina SBS summary30:30-Limits33:00-Multiplexing and Demultiplexing
Colour mixing in PHRED spaceChapters:00:00 Nucleotides level 209:00 Cycles12:00 Not-so-wobbly mutations15:30 Reducing colourspace22:30 Cutting connections28:14 Costs and PHRED- ClevaLabs on Illumina sequencing: https://www.youtube.c
Iuliia killed the stripey catChapters:00:00 Intro00:04:44 Stripes00:11:40 Sanger with colors00:17:20 CATCATCAT...00:33:20 Planes00:38:15 Genome assembly00:40:00 Transcriptomics01:01:00 Hi-C Why women are stripey: https://www.youtu
H+ ions and where we can find themChapters:00:00 Intro04:26 pH08:04 DNA Polymerase reaction with H+12:30 Nucleotides16:30 Homopolymer problem, but different19:30 PCR is mean to some bases24:30 AmpliSeq33:40 Today’s status38:58 cfDNA
If ligation is more your thing- Special Operations explaining SOLiD Sequencing: https://www.youtube.com/watch?v=YLT-DUeaLms- Writeup by the Apollo Institute on SOLiD Sequencing: https://apollo-institute.org/solid-sequencing/- Quick run-th
Iuliia and Daniel have fun with salt water and alcoholChapters:00:00:00 DIY DNA isolation 00:15:38 Directed evolution of EnzymesDNA isolation in your kitchen- Experiment during the episode: https://www.instructables.com/How-to-Extract-Y
Daniel and Iuliia are confused about fireflies- Scientific american on how fireflies create light:https://www.scientificamerican.com/article/how-and-why-do-fireflies/- A small breakdown of a typical Pyrosequencing workflow:https://www.new
Iuliia is confused about RNA and Daniel doesn’t know the size of a DNA nucleotideChapters:00:00-Intro02:00-DNA08:27-Enzymes13:05-RNAs, tRNAs16:18-mRNAs, µRNAs, lncRNAs, circRNAs21:35-Wobble23:30-PCR33:20-Sanger sequencing I36:30-Gel
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Teaser

The Explain Podcast hosts are bioinformatician Daniel and scientific coordinator Iuliia.We aim to EXPLAIN key next generation sequencing techniques and how these techniques contribute to our everyday life.The Podcast Explain is for everyone in
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