Mixing up long and cheapChapters:00:00:00 Intro00:03:30 Florians history00:13:50 Q&A Ressources for Bioinformatics00:27:00 Long-read-tools.org00:35:42 ONT || PacBio00:57:00 Storage01:09:00 OutroUp-to-date, raw prices:https://albertv
Rolling in circles to make DNA yarnsChapters:00:00 Bioinformatics Upper Crust Tools04:04 STAR05:00 MGI15:00 Daniels first contact20:00 Brief Market Overview22:30 Short read market26:00 DFG High Costs Sequencing Projects Application29:
Chapters:00:00 Gambling15:00 Bubbles30:00 Holes57:30 One more thing1:07:00 Finale1:08:00 Finale of season 1!Eric Chow explaining single cell sequencing: https://www.youtube.com/watch?v=k9VFNLLQP8cOverview of things to consider: http
Getting the most out of MachinesChapters:00:00 PhiX14:30 low complexity19:30 UMIs32:10 FastQC43:00 MultiQC56:40 PycoQCPhiX concentrations for loading a validation run:https://knowledge.illumina.com/instrumentation/general/instrumenta
Zipping rounds in zeptolitersChapters:00:00 Intro 07:00 Laser09:00 Zeptoliters13:30 Polymerase speed19:00 Error rate22:00 SMRT Cell25:45 ccs30:00 Fragment size34:30 Megaruptor38:20 Data flow47:00 SVs53:00 MAS Seq- Starter: https
Glued DNA bridges and rainbow clustersChapters:00:00-Flowcells03:40-DNA glued to glass09:00-Bridge amplification15:50-Cluster density and patterned Flowcells28:30-Illumina SBS summary30:30-Limits33:00-Multiplexing and Demultiplexing
H+ ions and where we can find themChapters:00:00 Intro04:26 pH08:04 DNA Polymerase reaction with H+12:30 Nucleotides16:30 Homopolymer problem, but different19:30 PCR is mean to some bases24:30 AmpliSeq33:40 Today’s status38:58 cfDNA
If ligation is more your thing- Special Operations explaining SOLiD Sequencing: https://www.youtube.com/watch?v=YLT-DUeaLms- Writeup by the Apollo Institute on SOLiD Sequencing: https://apollo-institute.org/solid-sequencing/- Quick run-th
Iuliia and Daniel have fun with salt water and alcoholChapters:00:00:00 DIY DNA isolation 00:15:38 Directed evolution of EnzymesDNA isolation in your kitchen- Experiment during the episode: https://www.instructables.com/How-to-Extract-Y
Daniel and Iuliia are confused about fireflies- Scientific american on how fireflies create light:https://www.scientificamerican.com/article/how-and-why-do-fireflies/- A small breakdown of a typical Pyrosequencing workflow:https://www.new
Iuliia is confused about RNA and Daniel doesn’t know the size of a DNA nucleotideChapters:00:00-Intro02:00-DNA08:27-Enzymes13:05-RNAs, tRNAs16:18-mRNAs, µRNAs, lncRNAs, circRNAs21:35-Wobble23:30-PCR33:20-Sanger sequencing I36:30-Gel
The Explain Podcast hosts are bioinformatician Daniel and scientific coordinator Iuliia.We aim to EXPLAIN key next generation sequencing techniques and how these techniques contribute to our everyday life.The Podcast Explain is for everyone in