Episodes of Biotech knowledge center

Oncolytic Virus in vitro Validation StudyOncolytic virus is engineered by homologous recombination from parental virus. The recombination rate is low and parental virus has growth superiority. In order to get purified engineered oncolytic viru

Oncolytic Virus Basis

Oncolytic Virotherapy Development for Combination Therapy with Cancer Vaccines

Oncolytic Virus BasisThe strategy implementation of immunotherapeutic for the treatment of cancer has gained prominence over the past decade in preclinical development and clinical practice. Traditional oncological treatments, including surger

Cytokine/Chemokine-expressing Oncolytic Virus

Oncolytic Virotherapy Development for Combination Therapy with Cancer VaccinesOncolytic virotherapy (OV) represents an exciting new area of cancer treatment by exploiting a virus's ability to selectively replicate and kill tumor tissue while s

Oncolytic Virus Efficacy Study

Cytokine/Chemokine-expressing Oncolytic VirusGenetic therapies, such as transfected viral vectors and immune cells, continue to display clinical responses as systemically deliverable and targeted therapeutics, with the first such strategy havi

Cytokine/Chemokine-expressing Oncolytic Virus

Antitumor effect of oncolytic virusThe antitumor effect of oncolytic virus can be analyzed by calculating the tumor size, tumor weight or through in vivo imaging, such as bioluminescence imaging (BLI) and position emission tomography (PET).

Oncolytic Virus in vitro Validation Study

Example 1: CCL5 Expressing Oncolytic VirusThe chemokine CCL5 is a RANTES, which is able to regulate upon activation and normal T cell expressed. It plays an important part in several human diseases and binds to three receptors: CCR1, CCR3, and

May 8th, 2023 8m 29s

Plaque Purification AssayOncolytic virus is engineered by homologous recombination from parental virus. The recombination rate is low and parental virus has growth superiority. In order to get purified engineered oncolytic virus, several round

Combination Therapy of Oncolytic Virus with Conventional Radiotherapy

May 8th, 2023 10m 9s

Combination Therapy of Oncolytic Virus with Conventional RadiotherapyNowadays, overcoming malignant tumors is still a major research topic in the medical community. The current methods of treating tumors are mainly surgery, chemotherapy and ra

messenger RNA

Apr 6th, 2023 12m 12s

With the significant progressing of scientific research and numerous ongoing pre-clinical development, messenger RNA has become a promising therapeutics modality for various indications including cancers and infectious diseases.https://mrna.c

2-Thiouridine Modification

5-Methylcytidine Modification

tRNA with s²U modifications shows a more stable structure and resistance against extreme thermophiles. Lack of the s²U modifications will result in growth defects, sensitivity to high temperatures, even protein misfolding in bacteria. Abnormali

6-Thioguanosine-Containing Cap

5-Methylcytidine (m⁵C), also called 5-methyl-cytidine, is a modified nucleoside derived from 5-methylcytosine (5mC), in which a methyl group is linked to the C-5 in the 6-atom ring of cytosine base catalyzed by DNA methyltransferase. 5mC has be

The classical cap structure is a 7-methylguanosine moiety connected to the mRNA via a triphosphate linkage at 5'-end (m7GpppG or m7G). It can be recognized by several specialized proteins involved in mRNA transport, translation, translational r

Actinomycin D

actinomycin d mRNA stability

Methods of mRNA stability tests such as transcription inhibition initially are proved most amenable for high-throughput technologies. Actinomycin D is a fast-acting DNA intercalator which is widely used as a transcription inhibitor to achieve m

Actinomycin D-based Method

Actinomycin D-based method as an easy approach has several advantages, for example, it does not require the construction and introduction of exogenous genes into cells. Although this method does not permit quantitative estimates of mRNA stabili

define mRNA

Custom iPS Cell Reprogramming by mRNA

Messenger ribonucleic acid (mRNA), a type of RNA molecule that can be found in various cells. In general, the structure of mRNA is often composed of a 5' cap, two untranslated regions (5' UTR and 3' UTR), a coding domain, a 3' poly(A) tail. mRN

mRNA capping

Apr 6th, 2023 12m 12s

The mRNA 5ʹ cap regulates splicing, nuclear output, resistance to exonuclease degradation, and initiation of translation. In short, 5' cap has the following functions:Regulation of nuclear export;Prevention of degradation by exonucleases;Pr

IVT mRNA

Mar 5th, 2023 12m 12s

With the development of IVT methods, synthetic mRNA has become an emerging class of gene therapy.https://mrna.creative-biolabs.com/custom-ivt-synthesis-of-mrna.htm

Chemical Synthesis of mRNA

Non-integrative mRNA reprogramming has proven to be the most efficient and clinically-relevant method for human induced pluripotent stem cells (iPSCs) reprogramming.https://mrna.creative-biolabs.com/custom-ips-cell-reprogramming-by-mrna.htm

Mar 5th, 2023 12m 12s

Chemical synthesized RNA can be used in the development of mRNA vaccines and genome editing tools, as well as the development of mRNA amplification-based diagnostics.https://mrna.creative-biolabs.com/custom-chemical-synthesis-of-mrna.htm

Chemical Synthesis of mRNA

Modified mRNAs can readily be obtained from any DNA template using RNA polymerases in a short time.https://mrna.creative-biolabs.com/custom-chemical-synthesis-of-mrna.htm

mRNA Products

In Vitro Transcription Vector

reagents for mRNA delivery, genome editing mRNA, reporter gene mRNA, growth factor mRNA, mRNA kits, enzymes for mRNA research, and nucleotide products for mRNA researchhttps://mrna.creative-biolabs.com/category-mrna-products-443.htm

In vitro transcription (IVT) is an elegant way to obtain highly pure and uniform mRNA oligonucleotides of lengths ranging from about 15 to several thousand nucleotides.https://mrna.creative-biolabs.com/category-in-vitro-transcription-vector-45

Cytokine mRNA