The fundamentals of the polymerase chain reaction (PCR) and how to calculate the specific concentration or molarity of the reaction elements required to perform a PCR reaction. Speaker: Art Braden, Ph.D., Roche Diagnostics

Leading the Pack with Molecular Quantitation on the LightCycler® 2.0 Instrument

Jul 18th, 2012 11m 57s

Areas of discussion include Chromosome Structure, Workflow of molecular testing, Biological Sample, Building a Real-Time PCR Reaction, Absolute Quantification Analysis, Automated Quantification Analysis, Data Analysis, Statistical Analysis.Spe

Molecular Stats: Limit of Detection & Limit of Quantification

Jul 18th, 2012 14m 22s

Overview of Limit of Detection (LoD) and Limit of Quantification (LoQ) as important analytical measurements in the performance of molecular assay

Molecular Stats: Prevalence and Positive Predictive Value

Jul 18th, 2012 10m 15s

Overview of how disease prevalence effects the concept of positive predictive value of a diagnostic test and also how changes in performance characteristics can affect the positive predictive value.

Molecular Stats: Correlation and Method Comparison

Jun 22nd, 2012 16m 18s

Method comparisons are a way to determine if a new method can replace an old method by confirming that the two tests sufficiently agree. This agreement is assessed based on visual and statistical parameters, which are discussed in the presentat

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